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Adipose Tissue Protocols

  • Book
  • © 2008

Overview

Editors:
  1. Kaiping Yang

    1. The University of Western Ontario, London, Canada

  • An indispensable, all-in-one manual for those interested in conducting any aspect of adipose tissue research
  • Collects all essential techniques with readily reproducible, step-by-step protocols to ensure success
  • Provides the most up-to-date methods in clear detail
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 456)

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About this book

Since the publication of the first edition, the techniques and experimental approaches employed in the study of adipose tissue have undergone radical expansion and revision. In Adipose Tissue Protocols: Second Edition, several updated chapters are combined with many new contributions from internationally recognized experts covering both conventional and state-of-the-art methodologies as well as whole animal, cellular and molecular approaches which have emerged in or been extended to adipose tissue research over the past few years. As a volume in the highly successful Methods in Molecular Biology™ series, each chapter includes an introduction to its subject matter, a list of necessary materials, step-by-step, readily reproducible laboratory protocols, and a Notes section, which provides invaluable tips and tricks to safeguard against potential mishaps and pitfalls.

Cutting-edge and comprehensive, Adipose Tissue Protocols: Second Edition is an essential manual for newcomers tothis burgeoning area as well as a valuable addition to the laboratories and offices of more experienced researchers.

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Overview of Adipose Tissue and Its Role in Obesity and Metabolic Disorders

    • Gema Frühbeck
    Pages 1-22

  3. Choosing an Adipose Tissue Depot for Sampling

    • Louis Casteilla, Luc Pénicaud, Béatrice Cousin, Denis Calise
    Pages 23-38

  4. Application of Imaging and Other Noninvasive Techniques in Determining Adipose Tissue Mass

    • Wei Shen, Jun Chen
    Pages 39-54

  5. Generation of Adipose Tissue-Specific Transgenic Mouse Models

    • Xian-Cheng Jiang
    Pages 55-64

  6. Angiogenesis in Adipose Tissue

    • Ebba Brakenhielm, Yihai Cao
    Pages 65-81

  7. Adipose Organ Nerves Revealed by Immunohistochemistry#

    • Antonio Giordano, Andrea Frontini, Saverio Cinti
    Pages 83-95

  8. Human Adipose Tissue Blood Flow and Micromanipulation of Human Subcutaneous Blood Flow

    • Gijs H. Goossens, Fredrik Karpe
    Pages 97-107

  9. Studies of Thermogenesis and Mitochondrial Function in Adipose Tissues

    • Barbara Cannon, Jan Nedergaard
    Pages 109-121

  10. Application of Lipidomics and Metabolomics to the Study of Adipose Tissue

    • Ismo Mattila, Tuulikki Seppänen-Laakso, Tapani Suortti, Matej Orešič
    Pages 123-130

  11. Applications of Proteomics to the Study of Adipose Tissue

    • Sonja Hess, Xiaoli Chen
    Pages 131-140

  12. Application of DNA Microarray to the Study of Human Adipose Tissue/Cells

    • Paska A. Permana, Saraswathy Nair, Yong-Ho Lee
    Pages 141-154

  13. Differentiation of Adipose Stem Cells

    • Bruce A. Bunnell, Bradley T. Estes, Farshid Guilak, Jeffrey M. Gimble
    Pages 155-171

  14. Methods That Resolve Different Contributions of Clonal Expansion to Adipogenesis in 3T3-L1 and C3H10T1/2 Cells

    • Colin R. Jefcoate, Suqing Wang, Xueqing Liu
    Pages 173-193

  15. Explant Cultures of White Adipose Tissue

    • Sébastien Thalmann, Cristiana E. Juge-Aubry, Christoph A. Meier
    Pages 195-199

  16. Isolation and Culture of Preadipocytes from Rodent White Adipose Tissue

    • Dorothy B. Hausman, Hea Jin Park, Gary J. Hausman
    Pages 201-219

  17. Flow Cytometry on the Stromal-Vascular Fraction of White Adipose Tissue

    • Danett K. Brake, C. Wayne Smith
    Pages 221-229

  18. Application of Electrophoretic Mobility Shift Assay and Chromatin Immunoprecipitation in the Study of Transcription in Adipose Cells

    • Melina M. Musri, Ramon Gomis, Marcelina Parrizas
    Pages 231-247

  19. Application of RNA Interference Techniques to Adipose Cell Cultures

    • Shigeki Shimba
    Pages 249-257

  20. RNA Isolation and Real-Time Quantitative RT-PCR

    • Haiyan Guan, Kaiping Yang
    Pages 259-270
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Editors and Affiliations

  • The University of Western Ontario, London, Canada

    Kaiping Yang

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Bibliographic Information

  • Book Title: Adipose Tissue Protocols

  • Editors: Kaiping Yang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-245-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-916-1Published: 06 June 2008

  • Softcover ISBN: 978-1-61737-844-7Published: 01 December 2010

  • eBook ISBN: 978-1-59745-245-8Published: 31 May 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 335

  • Topics: Cell Biology, Human Physiology, Endocrinology, Molecular Medicine

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