Table 1 Primer sequences and annealing temperatures for PCR
Primer set  | Position  | Annealing temperature  | 
|---|---|---|
β-catenin exon3  | ||
F:GTC TGA GGA GCA GCT TCA GT  | 77–96  | 56°C  | 
R:CAT TAG TGG GAT GAG CAG CA  | 559–578  | |
Axin  | ||
F1:GCG GGA CAG ATT GAT TCA CT  | 28–47  | 54°C  | 
R1:TCG GCA GGT ATC CAG ATA TG  | 811–830  | |
F2:TCT GTA GTG ACC AGA GCT CT  | 771–790  | 54°C  | 
R2:GAC GAT GGA TCG CCG TCC T  | 1380–1398  | |
F3:AGT TCG CGG AGG AGC TCA T  | 1272–1290  | 54°C  | 
R3:CCT CAA TGA TCC ACT GCA TG  | 2011–2030  | |
F4:AGG ATG CGG AGA AGA ACC AG  | 1986–2005  | 54°C  | 
R4:TCC TGA GTA CGA GGT CAT CT  | 2770–2789  |