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Isolation and differentiation of endometrial mesenchymal stem cells from Arabian mares

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This study aimed to extract and isolate endometrial stromal cells from Arabian mares and investigate their growth and differentiation potential. Endometrial biopsies were obtained from three healthy 6-year-old Arabian mares using a standardized, minimally invasive protocol. The isolated cells were characterized using flow cytometry and differentiation analysis. Flow cytometry revealed mesenchymal markers CD90 (95.2%) and CD105 (97.4%) and hematopoietic markers CD34 (1.17%) and CD45 (0.339%). The cells exhibited differentiation potential into adipocytes, osteoblasts, and chondrocytes. The findings suggest that endometrial cells from Arabian mares represent a promising autologous source of MSCs, particularly suited for regenerative applications in musculoskeletal disorders.

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Data sharing is not applicable to this article as no new data were created or analyzed in this study.

Notes

  1. Bone marrow–derived mesenchymal stem cells.

  2. Adipose tissue–derived mesenchymal stem cells.

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Acknowledgements

The authors of this article express their gratitude to the Research and Technology Vice-Chancellor of Shahid Chamran University of Ahvaz for their financial support.

Funding

This work was supported by the Shahid Chamran University of Ahvaz (grant number 1401).

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Authors and Affiliations

  1. Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Mohammadreza Sabzpoosh & Saad Gooraninejad

  2. Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Elham Hoveizi

Authors
  1. Mohammadreza Sabzpoosh
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  2. Elham Hoveizi
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  3. Saad Gooraninejad
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Contributions

Mohammadreza Sabzpoosh: contributed to writing—original draft, data and statistical analysis, methodology, and software. Elham Hoveizi: responsible for the overall supervision, conceptualization, and project administration. Saad Gooraninejad: contributed to conception and writing—review and editing. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Elham Hoveizi.

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Ethics approval

All animal protocols were approved by and performed in accordance with the Institutional Animal Ethics Committee of the Shahid Chamran University of Ahvaz (protocol number EE/1401.2.24.204108/scu.ac.ir). Also, all protocols complied with ARRIVE guidelines, and all the procedures strictly followed the Animal Scientific Procedures Act (1986).

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The authors declare no competing interests.

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Sabzpoosh, M., Hoveizi, E. & Gooraninejad, S. Isolation and differentiation of endometrial mesenchymal stem cells from Arabian mares. In Vitro Cell.Dev.Biol.-Animal (2025). https://doi.org/10.1007/s11626-025-01103-5

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  • DOI: https://doi.org/10.1007/s11626-025-01103-5

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