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Permissive infection of murine osteoblastic MC3T3-E1 cells by goatpox virus: establishing a novel cell model for mechanistic studies of host-pathogen interactions

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Goatpox is a highly contagious viral disease that poses a significant threat to the health of goats and other small ruminants. Here, we report for the first time that the murine osteoblastic cell line MC3T3-E1 supports productive GTPV infection, as demonstrated by Western blotting, indirect immunofluorescence assay, and flow cytometry. RNA sequencing revealed thousands of differentially expressed genes (DEGs). Signaling pathways and functions of the DEGs were analyzed using Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes. Real-time quantitative PCR further confirmed the expression of several DEGs. The present study extends the host range for GTPV replication and mechanistic investigations.

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Change history

  • 02 December 2025

    Typo in affiliation of author Huibao Wang updated.

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Funding

This work was supported by the Applied Basic Research Project of Qinghai Province, China (2024-ZJ-721), and the National Natural Science Foundation of China (32302850 and 32460876).

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Author notes

  1. Huibao Wang, Lina Tong, Shanhui Ren, and Xiaolong Gao these authors contributed equally to this manuscript and share first authorship.

Authors and Affiliations

  1. School of Environment Engineering, Gansu Forestry Voctech University, Tianshui, 741020, P.R. China

    Huibao Wang

  2. College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai, 810016, P.R. China

    Lina Tong & Xiaolong Gao

  3. State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, P.R. China

    Hongqiang Zhang, Haotai Chen & Shanhui Ren

  4. Dingxi Vocational and Technical College, Dingxi, Gansu, 730500, P.R. China

    Fangping Wang

Authors
  1. Huibao Wang
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  2. Lina Tong
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  3. Hongqiang Zhang
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  4. Fangping Wang
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  5. Haotai Chen
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  6. Shanhui Ren
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Corresponding authors

Correspondence to Shanhui Ren or Xiaolong Gao.

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All viral infection experiments were performed in accordance with the guidelines of Qinghai University and the Lanzhou Veterinary Research Institute, CAAS.

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The authors declare no competing financial interests.

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Wang, H., Tong, L., Zhang, H. et al. Permissive infection of murine osteoblastic MC3T3-E1 cells by goatpox virus: establishing a novel cell model for mechanistic studies of host-pathogen interactions. Arch Virol 171, 9 (2026). https://doi.org/10.1007/s00705-025-06460-w

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