Isolation of Polysomal RNA for Microarray Analysis

Arava, Yoav

doi:10.1385/1-59259-364-X:79

Yoav Arava³

Part of the book series: Methods in Molecular Biology ((MIMB,volume 224))

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35 Citations

Abstractt

DNA microarrays have been used extensively in recent years to study mRNA expression profiles of different cell types under various growth conditions. These steady-state mRNA profiles provide a wealth of information about cellular functions and responses. However, they do not necessarily reflect the ultimate gene expression profile of a cell since the step of translation might lead to discrepancies between the mRNA profile and the profile of the actual functioning unit in the cell, the protein (e.g., 1,2).

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Authors and Affiliations

Department of Biochemistry, Stanford University School of Medicine, Palo Alto, CA
Yoav Arava

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Yoav Arava
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Editors and Affiliations

Laboratory of Genetics, NIMH/NHGRI, National Institutes of Health, Rockville, MD
Michael J. Brownstein
Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, St. Paul, MN
Arkady B. Khodursky

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Arava, Y. (2003). Isolation of Polysomal RNA for Microarray Analysis. In: Brownstein, M.J., Khodursky, A.B. (eds) Functional Genomics. Methods in Molecular Biology, vol 224. Humana Press. https://doi.org/10.1385/1-59259-364-X:79

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DOI: https://doi.org/10.1385/1-59259-364-X:79
Publisher Name: Humana Press
Print ISBN: 978-1-58829-291-9
Online ISBN: 978-1-59259-364-4
eBook Packages: Springer Protocols

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