Tomato TFT1 Is Required for PAMP-Triggered Immunity and Mutations that Prevent T3S Effector XopN from Binding to TFT1 Attenuate Xanthomonas Virulence
Figure 5
XopN residues 605–733 are important for TFT1 binding and contribute to XopN-dependent virulence in tomato.
(A) Growth of Xcv ΔxopN (vector) (grey bars), Xcv ΔxopN (XopN1–604-HA) (blue bars), or Xcv ΔxopN (XopN-HA) (black bars) in susceptible tomato VF36 leaves. Leaves were inoculated with a 1×105 CFU/mL suspension of bacteria. Number of bacteria in each leaf was quantified at 0, 6 and 8 DPI. Data points represent mean CFU/cm2 ± SD of three plants. Analysis was repeated at least three times. Vector = pVSP61. Different letters at day 8 indicate statistically significant (one-way analysis of variance and Tukey's HSD test, P<0.05) differences between the samples. (B) Phenotype of tomato leaves inoculated with strains described in (A). Leaves were photographed at 12 DPI. Similar phenotypes were observed in 3 independent experiments.