Tomato TFT1 Is Required for PAMP-Triggered Immunity and Mutations that Prevent T3S Effector XopN from Binding to TFT1 Attenuate Xanthomonas Virulence
Figure 2
Reduced TFT1 mRNA expression in VIGS tomato leaves promotes Xcv ΔhrpF and Xcv ΔxopN growth.
(A) Growth of Xcv, Xcv ΔhrpF, or Xcv ΔxopN in control (TRV2) and TFT1-silenced (TRV2-TFT1) susceptible VF36 tomato lines. Leaves were inoculated with 1×105 CFU/mL of pathogen. Bacterial growth was quantified at 0, 6, and 9 DPI. Data points represent mean CFU/cm2 ± SD of four plants. Asterisk indicates significant difference (t test, P<0.05) in the infected TRV2-TFT1 lines compared to the similarly infected TRV2 lines. (B) Relative PR-1b1 mRNA levels in 4 control (TRV2) and 4 TFT1-silenced (TRV2-TFT1) tomato lines. Total RNA isolated from infected leaves in (A) on day 6 was used for Q-PCR. Actin mRNA expression was used to normalize the expression value in each sample. Error bars indicate SD for four plants. Asterisk indicates significant difference (t test, P<0.05) in the infected TRV2-TFT1 lines compared to the similarly infected TRV2 lines.