Tomato TFT1 Is Required for PAMP-Triggered Immunity and Mutations that Prevent T3S Effector XopN from Binding to TFT1 Attenuate Xanthomonas Virulence
Figure 1
TFT1 is a PTI-induced gene in tomato.
(A) Susceptible VF36 tomato leaves were inoculated with 10 mM MgCl2 (white bars), 1×105 CFU/mL of Xcv (black bars), or Xcv ΔxopN (grey bars). (B) Susceptible VF36 tomato leaves were inoculated with 10 mM MgCl2 (white bars), 2×108 CFU/mL of Xcv (black bars), Xcv ΔhrpF (yellow bars), or Xcv ΔhrcV (green bars). Total RNA was isolated at 2, 4, 6, and 8 DPI (A) or 6 HPI (B). Q-PCR was performed to monitor TFT1 mRNA levels. Actin expression was used to normalize the expression value in each sample, and relative expression values were determined against the average value of the sample infiltrated with 10 mM MgCl2 at each time point. Error bars indicate SD for three (A) and four (B) plants. Asterisk indicates significant difference (t test, P<0.05) relative to the 10 mM MgCl2 control at 6 or 8 DPI (A) or 6 HPI (B).