Issue 10, 2009
From the journal:

Chemical Society Reviews

The fluorescent protein palette: tools for cellular imaging†

Richard N. Day*a  and  Michael W. Davidsonb  

* Corresponding authors

a Department of Cellular and Integrative Physiology, Indiana University School of Medicine, 635 Barnhill Dr., Indianapolis, USA
E-mail: [email protected]
Fax: +1 317 274 3318
Tel: +1 317 274 2166

b National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, 1800 E. Paul Dirac Dr., Tallahassee, USA

Abstract

This critical review provides an overview of the continually expanding family of fluorescent proteins (FPs) that have become essential tools for studies of cell biology and physiology. Here, we describe the characteristics of the genetically encoded fluorescent markers that now span the visible spectrum from deep blue to deep red. We identify some of the novel FPs that have unusual characteristics that make them useful reporters of the dynamic behaviors of proteins inside cells, and describe how many different optical methods can be combined with the FPs to provide quantitative measurements in living systems (227 references).

 

“If wood is rubbed with the Pulmo marinus, it will have all the appearance of being on fire; so much so, indeed, that a walking-stick, thus treated, will light the way like a torch” (translation of Pliny the Elder from John Bostock, 1855).

Graphical abstract: The fluorescent protein palette: tools for cellular imaging
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Article information

DOI
https://doi.org/10.1039/B901966A
Article type
Critical Review
Submitted
08 May 2009
First published
04 Aug 2009

Chem. Soc. Rev., 2009,38, 2887-2921

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The fluorescent protein palette: tools for cellular imaging

R. N. Day and M. W. Davidson, Chem. Soc. Rev., 2009, 38, 2887 DOI: 10.1039/B901966A

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