Abstract
We developed a nonradioactive fluorescence-activated cell sorting–based assay, called surface sensing of translation (SUnSET), which allows the monitoring and quantification of global protein synthesis in individual mammalian cells and in heterogeneous cell populations. We demonstrate here, using mouse dendritic and T cells as a model, that SUnSET offers a technical alternative to classical radioactive labeling methods for the study of mRNA translation and cellular activation.
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Acknowledgements
E.K.S. and G.C. are supported by fellowships from the Ministère de la Recherche and la Fondation pour la Recherche Médicale. This work is supported by grants to P.P. from Ligue National Contre le Cancer, the Human Frontier Science Program and the European Network of Excellence DC-THERA. We thank E. Gatti for useful discussions and manuscript proofreading and members of the Plateforme d'Imagerie Commune du Site de Luminy (PICsL) for expert technical assistance.
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E.K.S., G.C. and M.C. performed experiments. E.K.S. and P.P. designed experiments and wrote the paper.
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Schmidt, E., Clavarino, G., Ceppi, M. et al. SUnSET, a nonradioactive method to monitor protein synthesis. Nat Methods 6, 275–277 (2009). https://doi.org/10.1038/nmeth.1314
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DOI: https://doi.org/10.1038/nmeth.1314
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