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Localization of light-harvesting complex apoproteins in the chloroplast and cytoplasm during greening ofChlamydomonas reinhardtii at 38°C

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Assembly of the major light-harvesting complex (LHC II) and development of photosynthetic function were examined during the initial phase of thylakoid biogenesis inChlamydomonas reinhardtii cells at 38°C. Continuous monitoring of LHC II fluorescence showed that these processes were initiated immediately upon exposure of cells to light. However, mature-size apoproteins of LHC II (Lhcb) increased in amount in an alkali-soluble (non-membrane) fraction in parallel with the increase in the membrane fraction. Alkali-soluble Lhcb were not integrated into membranes when protein synthesis was inhibited, suggesting that they were not active intermediates in LHC II assembly, nor were they recovered in a purified chloroplast preparation. Immunocytochemical analysis of greening cells revealed Lhcb inside the chloroplast near the envelope and in clusters deeper in the organelle. Antibody binding also detected Lhcb in granules within vacuoles in the cytosol, and Lhcb were recovered in granules purified from greening cells. Our results suggest that the cytosolic granules serve as receptacles of Lhcb synthesized in excess of the amount that can be accommodated by thylakoid membrane formation within the plastid envelope.

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Abbreviations

LHC II:

light-harvesting complex associated with PS II

Lhcb:

apoproteins of LHC II

Pchlide:

protochlorophyllide

Rubisco:

ribulose 1,5-bisphosphate carboxylase/oxygenase

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  1. Richard A. White

    Present address: Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 1st Street SW, 55905-0001, Rochester, MN, USA

Authors and Affiliations

  1. Department of Botany, Arizona State University, 85287-1601, Tempe, AZ, USA

    Richard A. White, Gregory R. Wolfe, Yutaka Komine & J. Kenneth Hoober

  2. Center for the Study of Early Events in Photosynthesis, Arizona State University, 85287-1601, Tempe, AZ, USA

    Richard A. White, Gregory R. Wolfe, Yutaka Komine & J. Kenneth Hoober

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White, R.A., Wolfe, G.R., Komine, Y. et al. Localization of light-harvesting complex apoproteins in the chloroplast and cytoplasm during greening ofChlamydomonas reinhardtii at 38°C. Photosynth Res 47, 267–280 (1996). https://doi.org/10.1007/BF02184287

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