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Fidelity of conjugation in Saccharomyces cerevisiae

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An efficient method for the production of synchronous zygotes in Saccharomyces cerevisiae is described. Cells were synchronised under defined conditions in either an a, α mixed culture or by incubation of each mating type in cell-free medium in which cells of the opposite mating type had been grown. Synchronised cells were allowed to fuse under defined conditions on filter membranes. This method was used to test the fidelity of conjugation in S. cerevisiae. Under conditions where cells of a or α mating type were in contact with up to 6 cells of each of two strains of opposite mating type, less than 1 multiple mating in 104 diploid matings occurred. It is concluded that in sexual conjugation in S. cerevisiae some process distinct from cell contact restricts cell fusion to paired combinations of conjugant cells.

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Authors and Affiliations

  1. Department of Biology, McGill University, H3A 1B1, Montreal, Quebec, Canada

    David Rogers & Howard Bussey

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  1. David Rogers
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  2. Howard Bussey
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Communicated by F. Kaudewitz

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Rogers, D., Bussey, H. Fidelity of conjugation in Saccharomyces cerevisiae . Molec. Gen. Genet. 162, 173–182 (1978). https://doi.org/10.1007/BF00267874

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  • DOI: https://doi.org/10.1007/BF00267874

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