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Interaction of the Rous Sarcoma Virus Protein pp60src with the Cellular Proteins pp50 and pp90

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Retroviruses 4

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 123))

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Abstract

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Oncogenic retroviruses cause multiple and profound alterations in the morphology, metabolism, and growth control of cells. In most retrovirus infected cells, all of these complex changes in the cellular phenotype are mediated by a single virus-encoded gene product, referred to as the transforming protein. Rous sarcoma virus (RSV) has proven to be an ideal system for the analysis of events which occur following oncogenic transformation by retroviruses. RSV induces rapid sarcoma production after injection of virus in vivo and efficient and rapid transformation of cells in culture (HANAFUSA 1977). The transforming gene of RSV encodes a protein of Mr 60 000 which has been designated pp60src (Brugge and Erikson 1977; Purchio et al. 1978). Genetic studies of viruses encoding mutant src gene products which induce a partially transformed phenotype suggest that interactions between pp60src and multiple cellular targets are required to elicit a fully transformed phenotype (review, Sefton and Hunter 1984). This review will discuss one such interaction between pp60src and host cell proteins. This interaction occurs between newly synthesized molecules of pp60src and two cellular proteins of Mr 90 000 (pp90) and 50 000 (pp50). The kinetics and localization of this interaction suggest that the cellular pp90 and pp50 proteins are involved in some aspect of the processing of pp60src before it reaches its residence in the plasma membrane (Courtneidge and Bishop 1982; BRUGGE et al. 1983). pp90 and pp50 have also been shown to associate with many retrovirus-encoded transforming proteins other than pp60src (Lipsich et al. 1982; Adkins et al. 1982). This suggests that pp50 and pp90 may play a common role in the events which take place after transformation by at least one class of retrovirus transforming proteins.

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Authors and Affiliations

  1. Department of Microbiology, State University of New York at Stony Brook, Stony Brook, NY, 11794, USA

    J. S. Brugge

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  1. J. S. Brugge
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  1. School of Medicine, Department of Microbiology, University of Southern California, 2025 Zonal Avenue HMR 401, 90033, Los Angeles, CA, USA

    Peter K. Vogt

  2. The Wistar Institute, 36th Street at Spruce, 19104, Philadelphia, PA, USA

    Hilary Koprowski

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© 1986 Springer-Verlag Berlin Heidelberg

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Brugge, J.S. (1986). Interaction of the Rous Sarcoma Virus Protein pp60src with the Cellular Proteins pp50 and pp90. In: Vogt, P.K., Koprowski, H. (eds) Retroviruses 4. Current Topics in Microbiology and Immunology, vol 123. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70810-7_1

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  • DOI: https://doi.org/10.1007/978-3-642-70810-7_1

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