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. 2026:2991:163-173.
doi: 10.1007/978-1-0716-5005-9_11.

Biochemical Reconstitution of Replication-Coupled Cohesin Acetylation

Masashi Minamino  1   2 , Céline Bouchoux  1 , Frank Uhlmann  3
Affiliations

Biochemical Reconstitution of Replication-Coupled Cohesin Acetylation

Masashi Minamino et al. Methods Mol Biol. 2026.

Abstract

Concomitantly with DNA replication, the cohesin complex establishes cohesion between the newly synthesized sister chromatids. Cohesion establishment requires acetylation of the cohesin subunit Smc3 by the Eco1 acetyltransferase. Acetylation is tightly coupled to DNA replication and depends on (i) DNA replication factors, (ii) DNA structures that transiently form at replication forks, as well as (iii) auxiliary cohesin complex components. Here, we describe a protocol to study DNA replication-coupled cohesin acetylation using purified budding yeast proteins.

Keywords: Chromosome segregation; Cohesin acetylation; DNA replication; In vitro reconstitution; S. cerevisiae; Sister chromatid cohesion.

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References

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