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Analysis of Viral and Cellular MicroRNAs in EBV-Infected Cells

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Epstein Barr Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1532))

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  • 14 Citations

Abstract

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MicroRNAs are small, noncoding RNAs that posttranscriptionally regulate gene expression. The discovery of this relatively new mode of gene regulation as well as studies showing the prognostic value of viral and cellular miRNAs as biomarkers, such as in cancer progression, has stimulated the development of many methods to characterize miRNAs. EBV encodes 25 viral precursor microRNAs within its genome that are expressed during lytic and latent infection. In addition to viral miRNAs, EBV infection induces the expression of specific cellular oncogenic miRNAs, such as miR-155, miR-146a, miR-21, and others, that can contribute to the persistence of latently infected cells. This chapter describes several current techniques used to identify and detect the expression of viral and cellular miRNAs in EBV-infected cells.

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Acknowledgments

R.L.S. is supported by NIH grant K99-CA175181. The author thanks the current and former members of Dr. Bryan Cullen’s laboratory at Duke University, the members of Dr. Jack Keene’s lab at Duke University, and the members of Dr. Jay Nelson’s laboratory at Oregon Health and Science University for the discussions, troubleshooting, and optimizing protocols over the years.

Author information

Authors and Affiliations

  1. Vaccine and Gene Therapy Institute, Oregon Health and Science University, 505 NW 185th Avenue, Beaverton, OR, USA

    Rebecca L. Skalsky

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  1. Rebecca L. Skalsky
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Correspondence to Rebecca L. Skalsky .

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Editors and Affiliations

  1. University of Szeged, Szeged, Hungary

    Janos Minarovits

  2. University of Regensburg, Regensburg, Germany

    Hans Helmut Niller

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Skalsky, R.L. (2017). Analysis of Viral and Cellular MicroRNAs in EBV-Infected Cells. In: Minarovits, J., Niller, H. (eds) Epstein Barr Virus. Methods in Molecular Biology, vol 1532. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6655-4_9

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  • DOI: https://doi.org/10.1007/978-1-4939-6655-4_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6653-0

  • Online ISBN: 978-1-4939-6655-4

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