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Biochemical Reconstitution of Replication-Coupled Cohesin Acetylation

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SMC Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2991))

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Concomitantly with DNA replication, the cohesin complex establishes cohesion between the newly synthesized sister chromatids. Cohesion establishment requires acetylation of the cohesin subunit Smc3 by the Eco1 acetyltransferase. Acetylation is tightly coupled to DNA replication and depends on (i) DNA replication factors, (ii) DNA structures that transiently form at replication forks, as well as (iii) auxiliary cohesin complex components. Here, we describe a protocol to study DNA replication-coupled cohesin acetylation using purified budding yeast proteins.

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References

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Author information

Authors and Affiliations

  1. Chromosome Segregation Laboratory, The Francis Crick Institute, London, UK

    Masashi Minamino, Céline Bouchoux & Frank Uhlmann

  2. Research Division, Chugai Pharmaceutical Co. Ltd., Yokohama, Japan

    Masashi Minamino

Authors
  1. Masashi Minamino
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  2. Céline Bouchoux
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  3. Frank Uhlmann
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Corresponding author

Correspondence to Frank Uhlmann .

Editor information

Editors and Affiliations

  1. Department of Chromosome Science, National Institute of Genetics, Shizuoka, Japan

    Yasuto Murayama

  2. Waseda Institute for Advanced Study, Waseda University, Tokyo, Japan

    Yasutaka Kakui

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© 2026 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Minamino, M., Bouchoux, C., Uhlmann, F. (2026). Biochemical Reconstitution of Replication-Coupled Cohesin Acetylation. In: Murayama, Y., Kakui, Y. (eds) SMC Proteins. Methods in Molecular Biology, vol 2991. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-5005-9_11

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  • DOI: https://doi.org/10.1007/978-1-0716-5005-9_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-5004-2

  • Online ISBN: 978-1-0716-5005-9

  • eBook Packages: Springer Protocols

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