PARP inhibitor olaparib induces DNA damage and acts as a drug sensitizer in an in vitro model of canine hematopoietic cancer

Table 2 Missense SNVs identified in DNA-damage response genes of CLBL-1 and GL-1 cell lines

	See More Cell line	CDS position	Exon	Frequency of the alternate allele	VEP prediction	Fido-SNP prediction
BRCA1	GL-1	c.604 A > G	9	HE	Tolerated	Benign
		c.715G > A	10	HE	Tolerated	Benign
		c.1329 A > C	10	HE	Deleterious	Pathogenic
		c.3619 A > G	10	HE	Tolerated	Benign
		c.5186G > A	13	HE	Tolerated	Benign
BRCA2	CLBL-1	c.428 A > G^a	5	HE	Tolerated	Pathogenic
		c.1158T > G^a	10	HO	Tolerated	Benign
		c.2401 A > C	11	HE	Deleterious	Benign
		c.4304 A > G^a	11	HE	Tolerated	Benign
	GL-1	c.428 A > G^a	5	HE	Tolerated	Pathogenic
		c.1158T > G^a	10	HE	Tolerated	Benign
		c.4304 A > G^a	11	HE	Tolerated	Benign
		c.6686G > T	11	HE	Tolerated	Benign
TP53	CLBL-1	c.374 C > T	5	HE	Deleterious	Pathogenic
	CLBL-1	c.764G > A	8	HE	Tolerated*	Pathogenic
	GL-1	c.709 C > T	7	HO	Deleterious	Benign
ATM	CLBL-1	c.3431 A > T^a	25	HE	Tolerated	Benign
	CLBL-1	c.5267T > C^a	36	HO	Tolerated	Benign
	GL-1	c.3431 A > T^a	25	HO	Tolerated	Benign
	GL-1	c.5267T > C^a	36	HO	Tolerated	Benign
ATR	CLBL-1	c.539G > A	4	HO	Tolerated**	Pathogenic

^aSNVs shared by CLBL-1 and GL-1 cell lines; *SIFT(Sorting Intolerant From Tolerant) = 0.09; **SIFT = 0.05; HE: heterozygous; HO: homozygous

ISSN: 1746-6148